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Acta
Pharm. 2004 Sep;54(3):243-50. |
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Antimicrobial activity of grapefruit seed and
pulp ethanolic extract
Cvetnic Z,
Vladimir-Knezevic S.
Department of Microbiology, Faculty of Pharmacy and
Biochemistry, University of Zagreb, Croatia. zcvetnic@yahoo.com
Antibacterial and antifungal activity of ethanolic extract of
grapefruit (Citrus paradisi Macf., Rutaceae) seed and pulp was
examined against 20 bacterial and 10 yeast strains. The level of
antimicrobial effects was established using an in vitro agar
assay and standard broth dilution susceptibility test. The
contents of 3.92% of total polyphenols and 0.11% of flavonoids
were determined spectrometrically in crude ethanolic extract.
The presence of flavanones naringin and hesperidin in the
extract was confirmed by TLC analysis. Ethanolic extract
exibited the strongest antimicrobial effect against Salmonella
enteritidis (MIC 2.06%, m/V). Other tested bacteria and yeasts
were sensitive to extract concentrations ranging from 4.13% to
16.50% (m/V).
PMID: 15610620 [PubMed - indexed for MEDLINE]
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